Epitope-Tagged Pkhd1 Tracks the Processing, Secretion, and Localization of Fibrocystin
نویسندگان
چکیده
منابع مشابه
Tagged fibrocystin sheds its secrets.
Autosomal recessive polycystic kidney disease (ARPKD), a rare monogenic disorder with an incidence of 1:20,000 live births, is characterized by massive bilateral kidney enlargement due to cystic dilation of the renal tubules.1 In addition, liver cysts and hepatic fibrosis are also observed in patients with ARPKD. In neonates and infants, ARPKD manifests as severe kidney failure in utero, oligoh...
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15 صفحه اولVectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells.
The FLAG peptide, AspTyrLysAspAspAspAspLys, has been used as an epitope tag in a variety of cell types. The modification of the cytomegalovirus (CMV) promoter containing vector, pCMV5, to create two transient expression vectors designed for secretion and intracellular expression of FLAG-fusion proteins in mammalian cells is described. As a functional test, the bacterial alkaline phosphatase gen...
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The hepatitis C virus (HCV) viroporin p7 is crucial for production of infectious viral progeny. However, its role in the viral replication cycle remains incompletely understood, in part due to the poor availability of p7-specific antibodies. To circumvent this obstacle, we inserted two consecutive hemagglutinin (HA) epitope tags at its N terminus. HA-tagged p7 reduced peak virus titers ca. 10-f...
متن کاملthe comparative impact of prompts and recasts in processing instruction versus meaningful output-based instruction on efl learners’ writing accuracy
the purpose of the present study was to see which one of the two instruction-processing instruction (pi) and meaningful output based instruction (mobi) accompanied with prompt and recast- is more effective on efl learners’ writing accuracy. in order to homogenize the participants in term of language proficiency a preliminary english test (pet) was administrated between 74 intermediate students ...
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ژورنال
عنوان ژورنال: Journal of the American Society of Nephrology
سال: 2011
ISSN: 1046-6673,1533-3450
DOI: 10.1681/asn.2010111173